Storage and Handling
The long-term storage of peptides presents a different problem. Lyophilized peptides generally have excellent stabilities (in most cases, lyophilized powders can be stored for years at -10ºC or lower temperatures with little or no degradation). Peptides are often hygroscopic. The absorption of moisture may decrease the stability and reduce the overall product quality. To avoid this vial should always be tightly sealed and when preparing for use, peptide should be allowed to attain the room temperature before opening the vial.
Peptides in solution are much less stable than lyophilized peptides. For best results, follow these guidelines:
It is recommended that the stock solution be aliquoted upon arrival to prevent degradation caused by repeated thawing and freezing. Thaw only what is needed and the remainder of the stock should be stored dry at ?20 ° C. Maintain peptides in an oxygen-free environment as peptides containing cysteine, methionine, tryptophan, glutamine, and asparagine are susceptible to oxidation and have limited shelf life. For maximum stability, store the sample at -20°C in a buffer solution with pH 5 to 7.
Reconstitution of Lyophilized peptide
Peptides can have widely varying solubility properties, depending largely on their primary sequence. Many peptides dissolve easily in water, whereas some, especially those containing multiple hydrophobic amino acid residues, may not readily dissolve. As a general procedure, we recommend attempting to reconstitute peptides in sterile distilled water with sonication if necessary. If solubility is still a problem, addition of a small amount of dilute (approximately 10%) aqueous acetic acid (for basic peptides) or aqueous ammonia (for acidic peptides) may facilitate dissolution of the peptide. It may initially be desirable initially to determine solubility on an aliquot of the total sample. The buffer of choice should only be added after the peptide is fully in solution, since salts may promote aggregation and therefore create solubility problems.
All peptides are supplied with stringent analytical specifications, which include HPLC and mass spectrometry analysis to prove the desired purity and identity of peptide respectively. Amino acid analysis and peptide sequencing of the product can also be provided upon request at an additional cost.
BioConcept guarantees the synthesis of your peptide to meet all listed specifications. If you are not completely satisfied, will either credit your account, or remake your peptides at no additional charge. Notification of potential problem has to be made within 7 days of receipt of the peptide. We cannot guarantee that the peptide which we synthesized will work in your experiment; we can only guarantee the purity and the identity of your specified compound, synthesized by us by providing you with HPLC chromatogram and mass spectrum data.
Peptides are generally shipped within 4-6 weeks of receipt of the purchase order. All peptides will be supplied as lyophilized powder. Some time delivery of the peptide is not guaranteed on stipulated time due to any technical problem on that case BiooConcept will not be liable for your experimental delay.